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© Arkansas Pathology Associates 2010
Anatomical Specimens
Fixation of Tissue Specimens
Materials Needed
1. Gloves, lab coat, and eye protection
2. Tissue container which will hold the specimen and an adequate amount of fixative
3. 10% Neutral buffered formalin
4. Tissue requisition
5. Transport bag
Specimen Collection
1. Wear gloves, lab coat, and eye protection.
2. Properly label specimen container with patient’s name and source of specimen.
3. Collect tissue immediately from physician and place into container with at least an equal volume of fixative. Make
sure container does not leak.
4. If Lymphoma is clinically suspected for a lymph node specimen, place a small portion of the lymph node into
properly labeled container of MEMS solution and refrigerate until courier pickup. The remainder of the lymph node
should be placed in a properly labeled container of formalin.
5. Complete tissue requisition with the following information:
Patient’s name, age, date of birth, SSN, and gender
Address
Doctor’s office or hospital and phone number
Insurance information
Relevant clinical history
Source of tissue
Doctor’s signature
2. Place the requisition in the pouch outside the sealed bag.
3. Transport specimens to the laboratory by courier.
4. If specimens are to be mailed, contact the laboratory for instructions.
Stones for Analysis
Materials Needed
1. Gloves, lab coat, and eye protection
2. Tissue container which will hold the specimen
3. Tissue requisition
4. Transport bag
Specimen Collection
1. Wear gloves, lab coat, and eye protection.
2. Properly label specimen container with patient’s name and source of specimen.
3. Collect tissue from physician and place into container without fixative.
4. Complete tissue requisition with the following information:
Patient’s name, age, date of birth, SSN, and gender
Address
Doctor’s office or hospital and phone number
Insurance information
Relevant clinical history
Source of tissue
Doctor’s signature
Request “stone analysis” on the requisition
Specimen Collection
1. Wear gloves, lab coat, and eye protection.
2. Properly label specimen container with patient’s name and source of specimen.
3. Immediately after biopsy is taken, tissue should be divided as follows:
1 core of kidney in Zinc Formalin for Electron Microscopy
2 cores of kidney in Michel’s fixative for immunofluorescence
3 cores of kidney for routine processing in formalin
Transport Specimen
1. Place specimen in transport bag and seal.
Transport Specimen
1. Place specimen in transport bag and seal.
2. Place the requisition in the pouch outside the sealed bag.
3. Transport specimens to the laboratory by courier.
4. If specimens are to be mailed, contact the laboratory for instructions.
Kidney Biopsy
Materials Needed
1. Gloves, lab coat, and eye protection
2. Kidney Biopsy Kit
3. Tissue requisition
4. Transport bag
4. Complete tissue requisition with the following information:
Patient’s name, age, date of birth, SSN, and gender
Address
Doctor’s office or hospital and phone number
Insurance information
Relevant clinical history
Source of tissue
Doctor’s signature
Transport Specimen
1. Place specimen(s) in Kidney Needle Biopsy Kit and place kit in transport bag and seal.
2. Place the requisition in the pouch outside the sealed bag.
3. Transport specimens to the laboratory by courier.
Breast Prognostic Markers
Specimen Required
1. Formalin fixed paraffin embedded tissue block
2. 1-H&E slide per tissue block
3. Completed pathology report or completed Immunohistochemistry staining request form.
4. Provide prognostic information for breast tumors.